June 6–11, 1999
The 1999 course was hosted by the National Flow Cytometry Resource at Los Alamos National Laboratory. Roger Tsien delivered the special lecture on imaging and sorting protein–protein interactions.
Lectures
- Introduction and Safety OrientationJim Jett
- Introduction to Flow CytometryJohn Martin
- Fluorescence Spectroscopy for Flow CytometristsLarry Sklar
- Stains, Dyes, and Other MagicAlan Waggoner
- DNA Analysis by Flow Cytometry: An OverviewAlice Givan
- Data Analysis 101Jim Jett
- Breast Cancer Prognosis: Relevancy of Flow Cytometric S-Phase and DNA PloidyBruce Bagwell
- Cellular Nucleic Acid AnalysisCharles Goolsby
- ApoptosisDavid Hedley
- Proliferation Antigen AnalysisJake Jacobberger
- GFP - applications in flow and image cytometryDavid Galbraith
- Flow Cytometric Cell Physiology AssaysJim Freyer
- ImmunophenotypingCarl Stewart
- SortingJoe Trotter
- Chromosome Analysis and SortingScott Cram
- Bead Based Assays of Molecular AssembliesJohn Nolan
- Special lecture — Imaging and Sorting Protein-Protein Interactions and Gene ExpressionRoger Tsien
Labs
- Build a Flow CytometerMartin / Wilder
- Methods in ImmunophenotypingStewart
- DNA AnalysisGivan / Bagwell
- ApoptosisHedley / Crissman / Becker
- Chromosome Analysis and SortingCram / Bell-Prince
- Measurement of Specific Nucleic Acids in Single CellsGoolsby
- Sorting/GFPTrotter / Galbraith
- Cell Cycle Antigens/PhysiologyJacobberger / Freyer
- SpectroscopyAlan Waggoner, Larry Sklar
- Build A Flow CytometerJohn Martin, Mark Wilder
- Phase Sensitive Flow CytometryJohn Steinkamp, Harry Crissman, Joe Valdez
- Kinetic Cytometry/Bead-Based AssaysLarry Sklar, John Nolan
- SortingJoe Trotter, Joe Fawcett
- DNA Fragment SizingPeter Goodwin, Jim Werner, Erica Larson, Mei Yan, Stefan Burde, Robb Habbersett, Jim Jett
Course Faculty
Bruce Bagwell, C. Kevin Becker, L. Scott Cram, Harry Crissman, Jim Freyer, David Galbraith, Alice Givan, Peter Goodwin, Charles Goolsby, Robert Habbersett, David Hedley, Jake Jacobberger, Jim Jett, John Martin, John Nolan, Larry Sklar, John Steinkamp, Carl Stewart, Joe Trotter, Roger Tsien, Alan Waggoner, Mark Wilder
