June 19–25, 2010
The 33rd Annual Course was held at Bowdoin College and introduced multispectral imaging flow cytometry to the program. Mario Roederer delivered the special lecture.
Lectures
- Flow Cytometry Basics and ReproducibilityJoe Trotter, Ph.D
- Fluorescence in CytometryAlan Waggoner, Ph.D
- Data Analysis 101James H. Jett, Ph.D
- Strategies in Multicolor Flow CytometryCarleton C. Stewart, Ph.D
- The Cytometry GameC. Bruce Bagwell, MD, Ph.D
- DNA Analysis: The BasicsAlice L. Givan, Ph.D
- Time, Biochemistry, and Cell States: Role of Cytometry in Systems BiologyJames Jacobberger, Ph.D
- Sorting StrategiesWilliam Telford, Ph.D
- Cytometry of Apoptosis
- Functional AssaysMarc Golightly, Ph.D
- Molecular MedicineDavid Hedley, Ph.D
- Fluorescent Proteins and CytometryDavid Galbraith, Ph.D
- Chromosome Sorting and GenomicsL. Scott Cram, Ph.D
- Ignorance Is Not Bliss — What Do Your Cells Look Like? Multispectral Imaging Flow CytometryKathleen McGrath, Ph.D
- Tracking Cell Trafficking and FunctionKathy Muirhead, Ph.D
- Monitoring Immune Function by Flow CytometryPaul Wallace, Ph.D
- Staining Intracellular Antigens (Including Phospho-Epitopes) for Flow Cytometric AnalysisVince Shankey, Ph.D
- Special Lecture: The Past, Present, and Future of Single Cell AnalysisMario Roederer, Ph.D
- The Future of Flow Cytometry InstrumentationJames Freyer, Ph.D
Labs
- Build a Flow CytometerWilder, Jett, and Naivar
- Chromosome AnalysisCram and Sanders
- Multicolor ImmunophenotypingStewart and Stewart
- Functional AssaysGolightly
- Advanced SortingTrotter
- DNA AnalysisGivan
- Monitoring Cell Function and ProliferationMuirhead, Wallace, and Tario
- Fluorescent Protein Analysis and SortingHawley, Herbert, Lambert, and Galbraith
- Multiparametric Cell Cycle AnalysisJacobberger, Sramkoski, Bray, and Stefan
- Cytometry of Cell SignalingHedley, Shankey, and Chow
- Methods for Apoptosis DetectionTelford, Tamul, and Bradford
- Utilizing Multispectral Imaging Flow Cytometry to Integrate Flow Cytometric and Morphometric AnalysesMcGrath
- Breaking the Dimensionality BarrierBagwell and Hunsberger
Course Faculty
C. Bruce Bagwell, Jolene Bradford, Christopher Bray, Sue Chow, L. Scott Cram, Jim Freyer, David Galbraith, Alice L. Givan, Marc Golightly, Teresa Hawley, David Hedley, Benjamin Hunsberger, James H. Jett, Georgina Lambert, Kathleen McGrath, Kathy Muirhead, Mark Naivar, David Novo, Mario Roederer, Claire Sanders, Vince Shankey, R. Michael Sramkoski, Tammy Stefan, Carleton C. Stewart, Sigrid Stewart, Karen Tamul, Joe Tario, William Telford, Joseph Trotter, Alan Waggoner, Paul Wallace, Mark Wilder
